StoriesCCRC talk supplement
Follow this story
I am unable to talk about all of my findings in detail in 10 minutes, but here are additional methods, graphs and data.
This story is by default licensed under the
MIT License
Details ...Copyright (c) 2025 Christopher Prevost Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.
LD in cells with overexpression of NRF2
Quantified LD content in HEK-TtH cell lines overexpressing NRF2 constructs.
Chapter 1: Citations
Sulforaphane stabilizes NRF2
Cells treated with Sulforaphane have stabilized NRF2.
Chapter 2: Citations
LD content in sulforaphane treated cells
Treated multiple cell lines with sulforaphane and measured LD content. Cells that are treated with this drug have increased LD content.
Chapter 3: Citations
NRF2 does not increase LD content through autophagy
Treated cells with a combination of chloroquine (CQ) which inhibits autophagy as well as sulforaphane which stabilizes NRF2. From this one experiment I do not observe any inhibition of LD accumulation in the sulforaphane + CQ treated cells.
NOTE: There was significant cell death in both CQ treated cell lines and this is a single experiment. Will repeat this experiment in 3-MA treated cells which also inhibits autophagy but will hopefully have significantly less cell death.
Chapter 4: Citations
Credits
Share This Story!
We are fascinated by organelle dynamics and their intersection with disease pathology
Christopher Prevost is a PhD Candidate at the Kashatus Lab
I am the youngest of 8 children, have many nieces and nephews, as well as 5 great nephews. I am a fan of movies, cooking, craft beer, and LEGO. I began a PhD program in Biochemistry and Molecular Genetics at the University of Virginia in Fall of 2019. I then joined the lab of Dave Kashatus in January of 2020.
Story Timeline
You're all caught up!
New cards will be added here as they become available.
Story References
This story has no citations.
Mila's Corner
On-demand help, dynamic content & more
We are currently working on multiple features to make them available in the subsequent updates:
¯\_(ツ)_/¯
We are currently working on discovery panel.